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Assay Detail

CHEMBL2162634

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human N-terminal His8-tagged Mcl1 expressed in Escherichia coli BL21(DE3) assessed as inhibition of Fluorescein-labeled BID binding after 1 to 2 hrs by fluorescence polarization assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Structure-based design of potent Bcl-2/Bcl-xL inhibitors with strong in vivo antitumor activity.
Zhou H, Aguilar A, Chen J, Bai L, Liu L, Meagher JL, Yang CY, McEachern D, Cong X, Stuckey JA, Wang S.
J Med Chem (2012) 55 : 6149 -6161
PubMed 22747598 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.74 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2159742 1 8.30
CHEMBL2159744 1 7.43
CHEMBL2159743 1 4.50
CHEMBL2159736 1 -
CHEMBL2159735 1 -
CHEMBL376408 ABT 737 1.0 1 -
CHEMBL2159741 1 -
CHEMBL2159740 1 -
CHEMBL2159739 1 -
CHEMBL2159737 1 -
CHEMBL2063897 1 -
CHEMBL2159734 1 -
CHEMBL2159733 1 -
CHEMBL2063880 1 -
CHEMBL2159738 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2159742 IC50 = 5.0 nM 8.30
CHEMBL2159744 IC50 = 37.0 nM 7.43
CHEMBL2159743 IC50 = 32000.0 nM 4.50
CHEMBL2159736 IC50 > 10000.0 nM -
CHEMBL2159735 IC50 > 10000.0 nM -
CHEMBL376408 ABT 737 IC50 > 1000.0 nM -
CHEMBL2159741 IC50 > 2000.0 nM -
CHEMBL2159740 IC50 > 2000.0 nM -
CHEMBL2159739 IC50 > 2000.0 nM -
CHEMBL2159737 IC50 > 2000.0 nM -
CHEMBL2063897 IC50 > 2000.0 nM -
CHEMBL2159734 IC50 > 100000.0 nM -
CHEMBL2159733 IC50 > 100000.0 nM -
CHEMBL2063880 IC50 > 100000.0 nM -
CHEMBL2159738 IC50 > 10000.0 nM -