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Assay Detail

CHEMBL2166571

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human GPR40 expressed in Flp-InTM T-RexTM HEK293 cells assessed as stimulation of calcium mobilization
8
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Free fatty acid receptor 1 (CHEMBL4422)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Free fatty acid receptor 1 (FFA1/GPR40) agonists: mesylpropoxy appendage lowers lipophilicity and improves ADME properties.
Christiansen E, Due-Hansen ME, Urban C, Grundmann M, Schröder R, Hudson BD, Milligan G, Cawthorne MA, Kostenis E, Kassack MU, Ulven T.
J Med Chem (2012) 55 : 6624 -6628
PubMed 22724451 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 8 7.74 8.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2164242 1 8.04
CHEMBL2164239 1 8.03
CHEMBL2164241 1 7.75
CHEMBL1773261 1 7.73
CHEMBL2163923 2 7.66
CHEMBL2164240 1 7.63
CHEMBL1688473 1 7.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2164242 EC50 = 9.12 nM 8.04
CHEMBL2164239 EC50 = 9.333 nM 8.03
CHEMBL2164241 EC50 = 17.78 nM 7.75
CHEMBL1773261 EC50 = 18.62 nM 7.73
CHEMBL2163923 EC50 = 21.88 nM 7.66
CHEMBL2164240 EC50 = 23.44 nM 7.63
CHEMBL2163923 EC50 = 26.0 nM 7.58
CHEMBL1688473 EC50 = 34.67 nM 7.46