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Assay Detail

CHEMBL2167144

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of beta-glucocerebrosidase in human fibroblast lysate using 4-methylumbelliferyl beta-D-glycopyranoside as substrate by fluorimetric analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysosomal acid glucosylceramidase (CHEMBL2179)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Conformationally-locked N-glycosides with selective β-glucosidase inhibitory activity: identification of a new non-iminosugar-type pharmacological chaperone for Gaucher disease.
Castilla J, Rísquez R, Cruz D, Higaki K, Nanba E, Ohno K, Suzuki Y, Díaz Y, Ortiz Mellet C, García Fernández JM, Castillón S.
J Med Chem (2012) 55 : 6857 -6865
PubMed 22762530 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 5.17 5.39

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL153479 AMBROXOL 3.0 1 5.39
CHEMBL2164031 1 4.94

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL153479 AMBROXOL IC50 = 4100.0 nM 5.39
CHEMBL2164031 IC50 = 11400.0 nM 4.94