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Assay Detail

CHEMBL2167614

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]-o-CRF from CRFR1 in rat frontal cortex after 2 hrs by gamma counter
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Frontal cortex
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Corticotropin-releasing factor receptor 1 (CHEMBL4649)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

[18F](R)-5-chloro-1-(1-cyclopropyl-2-methoxyethyl)-3-(4-(2-fluoroethoxy)-2,5-dimethyl phenylamino)pyrazin-2(1H)-one: introduction of N3-phenylpyrazinones as potential CRF-R1 PET imaging agents.
Deskus JA, Dischino DD, Mattson RJ, Ditta JL, Parker MF, Denhart DJ, Zuev D, Huang H, Hartz RA, Ahuja VT, Wong H, Mattson GK, Molski TF, Grace JE, Zueva L, Nielsen JM, Dulac H, Li YW, Guaraldi M, Azure M, Onthank D, Hayes M, Wexler E, McDonald J, Lodge NJ, Bronson JJ, Macor JE.
Bioorg Med Chem Lett (2012) 22 : 6651 -6655
PubMed 23010264 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 8.46 9.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL559437 1 9.59
CHEMBL2165202 1 9.17
CHEMBL550685 1 9.06
CHEMBL2165196 1 8.62
CHEMBL2165195 1 8.39
CHEMBL2165201 1 8.37
CHEMBL2165197 1 8.30
CHEMBL2165198 1 8.27
CHEMBL2165200 1 8.11
CHEMBL2165199 1 6.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL559437 IC50 = 0.26 nM 9.59
CHEMBL2165202 IC50 = 0.68 nM 9.17
CHEMBL550685 IC50 = 0.88 nM 9.06
CHEMBL2165196 IC50 = 2.4 nM 8.62
CHEMBL2165195 IC50 = 4.1 nM 8.39
CHEMBL2165201 IC50 = 4.3 nM 8.37
CHEMBL2165197 IC50 = 5.0 nM 8.30
CHEMBL2165198 IC50 = 5.4 nM 8.27
CHEMBL2165200 IC50 = 7.8 nM 8.11
CHEMBL2165199 IC50 = 210.0 nM 6.68