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Assay Detail

CHEMBL2168484

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human NaV1.2 alpha subunit expressed in CHOK1 cells at -65 mV holding potential by patch clamp electrophysiological assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium channel protein type 2 subunit alpha (CHEMBL4187)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

3-Oxoisoindoline-1-carboxamides: potent, state-dependent blockers of voltage-gated sodium channel Na(V)1.7 with efficacy in rat pain models.
Macsari I, Besidski Y, Csjernyik G, Nilsson LI, Sandberg L, Yngve U, Ahlin K, Bueters T, Eriksson AB, Lund PE, Venyike E, Oerther S, Hygge Blakeman K, Luo L, Arvidsson PI.
J Med Chem (2012) 55 : 6866 -6880
PubMed 22770500 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.52 5.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL698 TETRACAINE 4.0 1 5.96
CHEMBL629 AMITRIPTYLINE 4.0 1 5.51
CHEMBL2164389 1 5.09
CHEMBL2164044 1 -
CHEMBL2164042 1 -
CHEMBL2164373 1 -
CHEMBL2164371 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL698 TETRACAINE IC50 = 1100.0 nM 5.96
CHEMBL629 AMITRIPTYLINE IC50 = 3100.0 nM 5.51
CHEMBL2164389 IC50 = 8100.0 nM 5.09
CHEMBL2164044 IC50 > 33000.0 nM -
CHEMBL2164042 IC50 > 33000.0 nM -
CHEMBL2164373 IC50 > 33000.0 nM -
CHEMBL2164371 IC50 > 33000.0 nM -