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Assay Detail

CHEMBL2168701

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]iodoMLA from rat cerebral cortex alpha7 nAChR at 50 nM incubated for 2 hrs by microplate scintillation assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Cerebral cortex
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor subunit alpha-7 (CHEMBL4980)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and nicotinic acetylcholine receptor in vitro and in vivo pharmacological properties of 2'-fluoro-3'-(substituted phenyl)deschloroepibatidine analogues of 2'-fluoro-3'-(4-nitrophenyl)deschloroepibatidine.
Ondachi P, Castro A, Luetje CW, Damaj MI, Mascarella SW, Navarro HA, Carroll FI.
J Med Chem (2012) 55 : 6512 -6522
PubMed 22742586 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 6.61 7.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1076903 VARENICLINE 4.0 1 7.49
CHEMBL2164667 1 5.73
CHEMBL2164666 1 -
CHEMBL2164665 1 -
CHEMBL2164664 1 -
CHEMBL372638 1 -
CHEMBL2164663 1 -
CHEMBL361465 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1076903 VARENICLINE Ki = 32.5 nM 7.49
CHEMBL2164667 Ki = 1870.0 nM 5.73
CHEMBL2164666 Ki > 2000.0 nM -
CHEMBL2164665 Ki > 2000.0 nM -
CHEMBL2164664 Ki > 2000.0 nM -
CHEMBL372638 Ki > 2000.0 nM -
CHEMBL2164663 Ki > 2000.0 nM -
CHEMBL361465 Ki > 2000.0 nM -