Assay Detail
Binding
CHEMBL2173253
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of N terminal hexahistidine-tag recombinant human LSD1 expressed in Escherichia coli BL21 (DE3) using histone H3 peptide as substrate preincubated for 5 mins before substrate addition measured for 30 mins by microplate reader analysis
4
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Lysine-specific histone demethylase 1A (CHEMBL6136) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Oncoepigenomics: making histone lysine methylation count.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 5.66 | 5.72 |
| Ki | 2 | 5.38 | 5.51 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1215658 | — | — | 2 | 5.72 |
| CHEMBL1797639 | — | — | 2 | 5.60 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1215658 | — | IC50 | = | 1900.0 | nM | 5.72 |
| CHEMBL1797639 | — | IC50 | = | 2500.0 | nM | 5.60 |
| CHEMBL1215658 | — | Ki | = | 3100.0 | nM | 5.51 |
| CHEMBL1797639 | — | Ki | = | 5700.0 | nM | 5.24 |