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Assay Detail

CHEMBL2175401

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human rhinovirus 14 recombinant N-terminal GST-tagged protease 3c expressed in Escherichia coli DH5alpha using LEVLFQGPSK(Fluoro)G as substrate after 1 hr by discontinuous heterogeneous fluorometric assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism rhinovirus B14
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

A simple solid phase, peptide-based fluorescent assay for the efficient and universal screening of HRV 3C protease inhibitors.
Schünemann K, Connelly S, Kowalczyk R, Sperry J, Wilson IA, Fraser JD, Brimble MA.
Bioorg Med Chem Lett (2012) 22 : 5018 -5024
PubMed 22763202 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 4.59 5.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL349148 2-Bromo-1,4-naphthoquinone 1 5.38
CHEMBL2172471 1 5.06
CHEMBL596082 1 4.28
CHEMBL2172472 1 4.20
CHEMBL2172473 1 4.03

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL349148 2-Bromo-1,4-naphthoquinone IC50 = 4200.0 nM 5.38
CHEMBL2172471 IC50 = 8800.0 nM 5.06
CHEMBL596082 IC50 = 53000.0 nM 4.28
CHEMBL2172472 IC50 = 62700.0 nM 4.20
CHEMBL2172473 IC50 = 92500.0 nM 4.03