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Assay Detail

CHEMBL2183271

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HIF1alpha in human HCT116 cells under hypoxic condition after 16 hrs by dual luciferase reporter gene assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HCT-116
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hypoxia-inducible factor 1-alpha (CHEMBL4261)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationship of (E)-phenoxyacrylic amide derivatives as hypoxia-inducible factor (HIF) 1α inhibitors.
Naik R, Won M, Kim BK, Xia Y, Choi HK, Jin G, Jung Y, Kim HM, Lee K.
J Med Chem (2012) 55 : 10564 -10571
PubMed 23153200 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 5.74 6.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2178399 1 6.92
CHEMBL2178407 1 6.13
CHEMBL2178416 1 6.09
CHEMBL2178409 1 5.98
CHEMBL2178404 1 5.97
CHEMBL2178414 1 5.93
CHEMBL2178419 1 5.87
CHEMBL2178403 1 5.87
CHEMBL2178417 1 5.74
CHEMBL2178405 1 5.73
CHEMBL2178410 1 5.66
CHEMBL2178415 1 5.62
CHEMBL427092 1 5.61
CHEMBL2177118 1 5.59
CHEMBL2178418 1 5.58
CHEMBL2178411 1 5.38
CHEMBL373396 1 5.30
CHEMBL2178408 1 5.22
CHEMBL2178406 1 4.80
CHEMBL2178412 1 -
CHEMBL2178413 1 -
CHEMBL2178402 1 -
CHEMBL2178401 1 -
CHEMBL2178400 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2178399 IC50 = 120.0 nM 6.92
CHEMBL2178407 IC50 = 740.0 nM 6.13
CHEMBL2178416 IC50 = 820.0 nM 6.09
CHEMBL2178409 IC50 = 1040.0 nM 5.98
CHEMBL2178404 IC50 = 1070.0 nM 5.97
CHEMBL2178414 IC50 = 1180.0 nM 5.93
CHEMBL2178419 IC50 = 1350.0 nM 5.87
CHEMBL2178403 IC50 = 1340.0 nM 5.87
CHEMBL2178417 IC50 = 1840.0 nM 5.74
CHEMBL2178405 IC50 = 1850.0 nM 5.73
CHEMBL2178410 IC50 = 2170.0 nM 5.66
CHEMBL2178415 IC50 = 2380.0 nM 5.62
CHEMBL427092 IC50 = 2440.0 nM 5.61
CHEMBL2177118 IC50 = 2540.0 nM 5.59
CHEMBL2178418 IC50 = 2620.0 nM 5.58
CHEMBL2178411 IC50 = 4120.0 nM 5.38
CHEMBL373396 IC50 = 5030.0 nM 5.30
CHEMBL2178408 IC50 = 6030.0 nM 5.22
CHEMBL2178406 IC50 = 15750.0 nM 4.80
CHEMBL2178412 IC50 > 20000.0 nM -
CHEMBL2178413 IC50 > 20000.0 nM -
CHEMBL2178402 IC50 > 20000.0 nM -
CHEMBL2178401 IC50 > 20000.0 nM -
CHEMBL2178400 IC50 > 20000.0 nM -