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Assay Detail

CHEMBL2185173

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Binding
Inhibition of NAALADase activity in human LNCaP cell membranes assessed as inhibition of [3H]NAG conversion to [3H]glutamate after 30 mins by liquid scintillation counting
12
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type LNCaP
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glutamate carboxypeptidase 2 (CHEMBL1892)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Radiofluorinated derivatives of 2-(phosphonomethyl)pentanedioic acid as inhibitors of prostate specific membrane antigen (PSMA) for the imaging of prostate cancer.
Graham K, Lesche R, Gromov AV, Böhnke N, Schäfer M, Hassfeld J, Dinkelborg L, Kettschau G.
J Med Chem (2012) 55 : 9510 -9520
PubMed 23025786 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 8.41 9.40
IC50 6 8.01 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL47009 2 9.40
CHEMBL2177507 2 8.85
CHEMBL2177508 2 8.59
CHEMBL2177509 2 8.51
CHEMBL2177510 2 7.66
CHEMBL2177506 2 7.44

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL47009 Ki = 0.4 nM 9.40
CHEMBL47009 IC50 = 1.1 nM 8.96
CHEMBL2177507 Ki = 1.4 nM 8.85
CHEMBL2177508 Ki = 2.6 nM 8.59
CHEMBL2177509 Ki = 3.1 nM 8.51
CHEMBL2177507 IC50 = 3.1 nM 8.51
CHEMBL2177508 IC50 = 6.8 nM 8.17
CHEMBL2177509 IC50 = 8.1 nM 8.09
CHEMBL2177510 Ki = 22.0 nM 7.66
CHEMBL2177506 Ki = 36.4 nM 7.44
CHEMBL2177510 IC50 = 55.9 nM 7.25
CHEMBL2177506 IC50 = 89.4 nM 7.05