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Assay Detail

CHEMBL2187464

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human alpha4beta2 nAChR expressed in human SH-EP1 cells by whole cell voltage clamp based electrophysiology method
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type SH-EP1
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907589)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Structure-activity studies of 7-heteroaryl-3-azabicyclo[3.3.1]non-6-enes: a novel class of highly potent nicotinic receptor ligands.
Breining SR, Melvin M, Bhatti BS, Byrd GD, Kiser MN, Hepler CD, Hooker DN, Zhang J, Reynolds LA, Benson LR, Fedorov NB, Sidach SS, Mitchener JP, Lucero LM, Lukas RJ, Whiteaker P, Yohannes D.
J Med Chem (2012) 55 : 9929 -9945
PubMed 23025891 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 7 6.12 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2177537 1 7.30
CHEMBL2177538 1 7.00
CHEMBL2177545 1 6.23
CHEMBL2177543 1 5.85
CHEMBL2177539 1 5.51
CHEMBL2177544 1 5.46
CHEMBL2177540 1 5.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2177537 EC50 = 50.0 nM 7.30
CHEMBL2177538 EC50 = 100.0 nM 7.00
CHEMBL2177545 EC50 = 590.0 nM 6.23
CHEMBL2177543 EC50 = 1400.0 nM 5.85
CHEMBL2177539 EC50 = 3100.0 nM 5.51
CHEMBL2177544 EC50 = 3500.0 nM 5.46
CHEMBL2177540 EC50 = 3500.0 nM 5.46