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Assay Detail

CHEMBL2187699

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of human recombinant PACE4 expressed in Drosophila S2 cells using pyroGlu-Arg-Val-Lys-Arg-methyl-coumaryl-7-amide as substrate at pH 6.5 after 60 mins by spectrofluorometric analysis
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type S2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proprotein convertase subtilisin/kexin type 6 (CHEMBL2951)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The Multi-Leu peptide inhibitor discriminates between PACE4 and furin and exhibits antiproliferative effects on prostate cancer cells.
Levesque C, Fugère M, Kwiatkowska A, Couture F, Desjardins R, Routhier S, Moussette P, Prahl A, Lammek B, Appel JR, Houghten RA, D'Anjou F, Dory YL, Neugebauer W, Day R.
J Med Chem (2012) 55 : 10501 -10511
PubMed 23126600 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 17 7.63 9.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2179430 1 9.47
CHEMBL2179835 1 9.40
CHEMBL2179836 1 8.96
CHEMBL2179429 1 8.89
CHEMBL2179431 1 8.70
CHEMBL2179428 1 8.15
CHEMBL2179434 1 7.75
CHEMBL2179832 1 7.72
CHEMBL2179435 1 7.66
CHEMBL2179833 1 7.64
CHEMBL2179436 1 7.16
CHEMBL2049156 1 6.62
CHEMBL2179437 1 6.52
CHEMBL2179438 1 5.86
CHEMBL2179432 1 5.80
CHEMBL2179433 1 5.80
CHEMBL2179427 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2179430 Ki = 0.34 nM 9.47
CHEMBL2179835 Ki = 0.4 nM 9.40
CHEMBL2179836 Ki = 1.1 nM 8.96
CHEMBL2179429 Ki = 1.3 nM 8.89
CHEMBL2179431 Ki = 2.0 nM 8.70
CHEMBL2179428 Ki = 7.0 nM 8.15
CHEMBL2179434 Ki = 18.0 nM 7.75
CHEMBL2179832 Ki = 19.0 nM 7.72
CHEMBL2179435 Ki = 22.0 nM 7.66
CHEMBL2179833 Ki = 23.0 nM 7.64
CHEMBL2179436 Ki = 70.0 nM 7.16
CHEMBL2049156 Ki = 240.0 nM 6.62
CHEMBL2179437 Ki = 300.0 nM 6.52
CHEMBL2179438 Ki = 1380.0 nM 5.86
CHEMBL2179432 Ki = 1600.0 nM 5.80
CHEMBL2179433 Ki = 1600.0 nM 5.80
CHEMBL2179427 Ki > 1000.0 nM -