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Assay Detail

CHEMBL2187733

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at mouse TGR5 expressed in HEK293 cells incubated for 5.5 hrs by CRE-driven luciferase reporter gene assay
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G-protein coupled bile acid receptor 1 (CHEMBL1255150)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Design, synthesis, and antidiabetic activity of 4-phenoxynicotinamide and 4-phenoxypyrimidine-5-carboxamide derivatives as potent and orally efficacious TGR5 agonists.
Duan H, Ning M, Chen X, Zou Q, Zhang L, Feng Y, Zhang L, Leng Y, Shen J.
J Med Chem (2012) 55 : 10475 -10489
PubMed 23148522 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 28 7.89 9.01

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2181229 1 9.01
CHEMBL2181227 1 8.70
CHEMBL2181228 1 8.68
CHEMBL2181230 1 8.64
CHEMBL2181226 1 8.21
CHEMBL2181225 1 7.82
CHEMBL2181239 1 7.75
CHEMBL2181235 1 7.62
CHEMBL2181223 1 7.58
CHEMBL2181221 1 7.52
CHEMBL2181256 1 7.52
CHEMBL2181222 1 7.51
CHEMBL2181240 1 7.51
CHEMBL2181238 1 7.18
CHEMBL2181236 1 7.12
CHEMBL2181237 1 -
CHEMBL2181234 1 -
CHEMBL2181233 1 -
CHEMBL2181232 1 -
CHEMBL2181231 1 -
CHEMBL2181257 1 -
CHEMBL2181255 1 -
CHEMBL2181254 1 -
CHEMBL2181253 1 -
CHEMBL2181252 1 -
CHEMBL2181251 1 -
CHEMBL2181224 1 -
CHEMBL2181220 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2181229 EC50 = 0.98 nM 9.01
CHEMBL2181227 EC50 = 2.0 nM 8.70
CHEMBL2181228 EC50 = 2.1 nM 8.68
CHEMBL2181230 EC50 = 2.3 nM 8.64
CHEMBL2181226 EC50 = 6.2 nM 8.21
CHEMBL2181225 EC50 = 15.0 nM 7.82
CHEMBL2181239 EC50 = 18.0 nM 7.75
CHEMBL2181235 EC50 = 24.0 nM 7.62
CHEMBL2181223 EC50 = 26.0 nM 7.58
CHEMBL2181221 EC50 = 30.0 nM 7.52
CHEMBL2181256 EC50 = 30.0 nM 7.52
CHEMBL2181222 EC50 = 31.0 nM 7.51
CHEMBL2181240 EC50 = 31.0 nM 7.51
CHEMBL2181238 EC50 = 66.0 nM 7.18
CHEMBL2181236 EC50 = 76.0 nM 7.12
CHEMBL2181237 EC50 - -
CHEMBL2181234 EC50 - -
CHEMBL2181233 EC50 - -
CHEMBL2181232 EC50 - -
CHEMBL2181231 EC50 - -
CHEMBL2181257 EC50 - -
CHEMBL2181255 EC50 - -
CHEMBL2181254 EC50 - -
CHEMBL2181253 EC50 - -
CHEMBL2181252 EC50 - -
CHEMBL2181251 EC50 - -
CHEMBL2181224 EC50 - -
CHEMBL2181220 EC50 - -