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Assay Detail

CHEMBL2188255

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of IDH1 R132C mutant overexpressed in human HT1080 cells assessed as inhibition of 2-hydroxyglutarate production after 48 hrs by LC/MS analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HT-1080
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Isocitrate dehydrogenase [NADP] cytoplasmic (CHEMBL2007625)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of the First Potent Inhibitors of Mutant IDH1 That Lower Tumor 2-HG in Vivo.
Popovici-Muller J, Saunders JO, Salituro FG, Travins JM, Yan S, Zhao F, Gross S, Dang L, Yen KE, Yang H, Straley KS, Jin S, Kunii K, Fantin VR, Zhang S, Pan Q, Shi D, Biller SA, Su SM.
ACS Med Chem Lett (2012) 3 : 850 -855
PubMed 24900389 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.70 7.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2180740 1 7.05
CHEMBL2180728 1 6.96
CHEMBL2180730 1 6.82
CHEMBL2180729 1 6.77
CHEMBL2180738 1 6.66
CHEMBL2180739 1 6.58
CHEMBL2180746 1 6.41
CHEMBL2180727 1 6.32

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2180740 IC50 = 90.0 nM 7.05
CHEMBL2180728 IC50 = 110.0 nM 6.96
CHEMBL2180730 IC50 = 150.0 nM 6.82
CHEMBL2180729 IC50 = 170.0 nM 6.77
CHEMBL2180738 IC50 = 220.0 nM 6.66
CHEMBL2180739 IC50 = 260.0 nM 6.58
CHEMBL2180746 IC50 = 390.0 nM 6.41
CHEMBL2180727 IC50 = 480.0 nM 6.32