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Assay Detail

CHEMBL2209121

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PRC2 complex of FLAG-EZH2, EED, SUZ12, AEBP2 and RbAp48 using histone H3 peptide (21 to 44) as substrate and [3H]SAM after 30 mins
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 6 — Multiple protein complex / RNA
Curated By Autocuration

Target

EZH2/SUZ12/EED/RBBP7/RBBP4 (CHEMBL3301388)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Identification of Potent, Selective, Cell-Active Inhibitors of the Histone Lysine Methyltransferase EZH2.
Verma SK, Tian X, LaFrance LV, Duquenne C, Suarez DP, Newlander KA, Romeril SP, Burgess JL, Grant SW, Brackley JA, Graves AP, Scherzer DA, Shu A, Thompson C, Ott HM, Aller GS, Machutta CA, Diaz E, Jiang Y, Johnson NW, Knight SD, Kruger RG, McCabe MT, Dhanak D, Tummino PJ, Creasy CL, Miller WH.
ACS Med Chem Lett (2012) 3 : 1091 -1096
PubMed 24900432 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 8.01 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2204997 1 9.22
CHEMBL2204995 1 8.92
CHEMBL2204998 1 8.10
CHEMBL2204996 1 7.85
CHEMBL2204999 1 7.13
CHEMBL1608462 1 6.83

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2204997 Ki = 0.6 nM 9.22
CHEMBL2204995 Ki = 1.2 nM 8.92
CHEMBL2204998 Ki = 7.9 nM 8.10
CHEMBL2204996 Ki = 14.0 nM 7.85
CHEMBL2204999 Ki = 74.0 nM 7.13
CHEMBL1608462 Ki = 149.0 nM 6.83