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Assay Detail

CHEMBL2210541

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of APN in human ES2 cell surface assessed as inhibition of L-Leu-p-nitroanilide substrate hydrolysis incubated for 5 mins before substrate addition by UV-Vis spectrophotometric analysis
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type ES2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aminopeptidase N (CHEMBL1907)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of Synthetic Aminopeptidase N/CD13 Inhibitors to Overcome Cancer Metastasis and Angiogenesis.
Su L, Cao J, Jia Y, Zhang X, Fang H, Xu W.
ACS Med Chem Lett (2012) 3 : 959 -964
PubMed 24900417 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.76 6.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2204959 1 6.38
CHEMBL2204943 1 6.32
CHEMBL2204950 1 6.10
CHEMBL2204954 1 6.04
CHEMBL2204947 1 6.03
CHEMBL2204949 1 5.96
CHEMBL2204953 1 5.82
CHEMBL2204951 1 5.52
CHEMBL2204945 1 5.19
CHEMBL2204952 1 5.16
CHEMBL29292 UBENIMEX 2.0 1 4.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2204959 IC50 = 420.0 nM 6.38
CHEMBL2204943 IC50 = 480.0 nM 6.32
CHEMBL2204950 IC50 = 790.0 nM 6.10
CHEMBL2204954 IC50 = 910.0 nM 6.04
CHEMBL2204947 IC50 = 940.0 nM 6.03
CHEMBL2204949 IC50 = 1100.0 nM 5.96
CHEMBL2204953 IC50 = 1500.0 nM 5.82
CHEMBL2204951 IC50 = 3000.0 nM 5.52
CHEMBL2204945 IC50 = 6400.0 nM 5.19
CHEMBL2204952 IC50 = 6900.0 nM 5.16
CHEMBL29292 UBENIMEX IC50 = 16000.0 nM 4.80