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Assay Detail

CHEMBL2215525

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of radioligand [3H]SP from human NK1 F264Y6.51 mutant expressed in HEK293 cells
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Substance-P receptor (CHEMBL249)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of a crucial amino acid in the helix position 6.51 of human tachykinin neurokinin 1 and 3 receptors contributing to the insurmountable mode of antagonism by dual NK₁/NK₃ antagonists.
Bissantz C, Bohnert C, Hoffmann T, Marcuz A, Schnider P, Malherbe P.
J Med Chem (2012) 55 : 5061 -5076
PubMed 22574973 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 8.96 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL16192 CP-96345 1 9.52
CHEMBL2203704 1 9.30
CHEMBL1471 APREPITANT 4.0 1 9.10
CHEMBL2203703 1 8.96
CHEMBL2203707 1 8.89
CHEMBL2203708 1 8.85
CHEMBL2203709 1 8.77
CHEMBL2203706 1 8.68
CHEMBL2203705 1 8.55
CHEMBL10188 TALNETANT 2.0 1 -
CHEMBL346178 OSANETANT 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL16192 CP-96345 Ki = 0.3 nM 9.52
CHEMBL2203704 Ki = 0.5 nM 9.30
CHEMBL1471 APREPITANT Ki = 0.8 nM 9.10
CHEMBL2203703 Ki = 1.1 nM 8.96
CHEMBL2203707 Ki = 1.3 nM 8.89
CHEMBL2203708 Ki = 1.4 nM 8.85
CHEMBL2203709 Ki = 1.7 nM 8.77
CHEMBL2203706 Ki = 2.1 nM 8.68
CHEMBL2203705 Ki = 2.8 nM 8.55
CHEMBL10188 TALNETANT Ki - -
CHEMBL346178 OSANETANT Ki - -