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Assay Detail

CHEMBL2320327

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Transactivation of GAL4-fused human PPARgamma ligand binding domain expressed in HepG2 cells after 20 hrs by luciferase reporter gene assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HepG2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor gamma (CHEMBL235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

New 2-(aryloxy)-3-phenylpropanoic acids as peroxisome proliferator-activated receptor α/γ dual agonists able to upregulate mitochondrial carnitine shuttle system gene expression.
Laghezza A, Pochetti G, Lavecchia A, Fracchiolla G, Faliti S, Piemontese L, Di Giovanni C, Iacobazzi V, Infantino V, Montanari R, Capelli D, Tortorella P, Loiodice F.
J Med Chem (2013) 56 : 60 -72
PubMed 23171045 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 17 6.06 7.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL121 ROSIGLITAZONE 4.0 1 7.41
CHEMBL2312713 1 6.34
CHEMBL191275 1 6.26
CHEMBL2312712 1 6.23
CHEMBL2312715 1 6.19
CHEMBL2312707 1 6.16
CHEMBL2312708 1 6.16
CHEMBL2312716 1 6.16
CHEMBL2312702 1 6.00
CHEMBL2312717 1 6.00
CHEMBL2312701 1 5.92
CHEMBL2312714 1 5.87
CHEMBL2312718 1 5.85
CHEMBL2312710 1 5.69
CHEMBL2312700 1 5.64
CHEMBL2312711 1 5.59
CHEMBL2312709 1 5.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL121 ROSIGLITAZONE EC50 = 39.0 nM 7.41
CHEMBL2312713 EC50 = 460.0 nM 6.34
CHEMBL191275 EC50 = 550.0 nM 6.26
CHEMBL2312712 EC50 = 590.0 nM 6.23
CHEMBL2312715 EC50 = 640.0 nM 6.19
CHEMBL2312707 EC50 = 700.0 nM 6.16
CHEMBL2312708 EC50 = 700.0 nM 6.16
CHEMBL2312716 EC50 = 690.0 nM 6.16
CHEMBL2312702 EC50 = 1000.0 nM 6.00
CHEMBL2312717 EC50 = 1000.0 nM 6.00
CHEMBL2312701 EC50 = 1200.0 nM 5.92
CHEMBL2312714 EC50 = 1360.0 nM 5.87
CHEMBL2312718 EC50 = 1400.0 nM 5.85
CHEMBL2312710 EC50 = 2040.0 nM 5.69
CHEMBL2312700 EC50 = 2300.0 nM 5.64
CHEMBL2312711 EC50 = 2540.0 nM 5.59
CHEMBL2312709 EC50 = 3100.0 nM 5.51