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Assay Detail

CHEMBL2320404

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at mouse EP1 receptor expressed in CHOK1 cells assessed as inhibition of 17PTPGE2-induced calcium influx
28
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin E2 receptor EP1 subtype (CHEMBL2181)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Development of an in vivo active, dual EP1 and EP3 selective antagonist based on a novel acyl sulfonamide bioisostere.
Downey JD, Saleh SA, Bridges TM, Morrison RD, Daniels JS, Lindsley CW, Breyer RM.
Bioorg Med Chem Lett (2013) 23 : 37 -41
PubMed 23218714 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 28 6.27 7.39

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2315055 2 7.39
CHEMBL1207972 2 7.32
CHEMBL2315056 2 7.27
CHEMBL2315059 2 6.67
CHEMBL2315057 2 6.67
CHEMBL2315049 2 6.52
CHEMBL2315052 2 6.35
CHEMBL2315046 2 6.26
CHEMBL2315048 2 6.04
CHEMBL2315050 2 5.86
CHEMBL2315053 2 5.66
CHEMBL2315054 2 5.58
CHEMBL2315051 2 5.36
CHEMBL2315047 2 4.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2315055 Kd = 40.7 nM 7.39
CHEMBL2315055 Kd = 40.74 nM 7.39
CHEMBL1207972 Kd = 47.9 nM 7.32
CHEMBL1207972 Kd = 47.86 nM 7.32
CHEMBL2315056 Kd = 53.7 nM 7.27
CHEMBL2315056 Kd = 56.23 nM 7.25
CHEMBL2315059 Kd = 214.0 nM 6.67
CHEMBL2315057 Kd = 214.0 nM 6.67
CHEMBL2315059 Kd = 213.8 nM 6.67
CHEMBL2315057 Kd = 213.8 nM 6.67
CHEMBL2315049 Kd = 302.0 nM 6.52
CHEMBL2315049 Kd = 302.0 nM 6.52
CHEMBL2315052 Kd = 446.68 nM 6.35
CHEMBL2315052 Kd = 447.0 nM 6.35
CHEMBL2315046 Kd = 549.54 nM 6.26
CHEMBL2315046 Kd = 549.0 nM 6.26
CHEMBL2315048 Kd = 912.01 nM 6.04
CHEMBL2315048 Kd = 912.0 nM 6.04
CHEMBL2315050 Kd = 1380.0 nM 5.86
CHEMBL2315050 Kd = 1380.38 nM 5.86
CHEMBL2315053 Kd = 2190.0 nM 5.66
CHEMBL2315053 Kd = 2187.76 nM 5.66
CHEMBL2315054 Kd = 2630.0 nM 5.58
CHEMBL2315054 Kd = 2630.27 nM 5.58
CHEMBL2315051 Kd = 4360.0 nM 5.36
CHEMBL2315051 Kd = 4365.16 nM 5.36
CHEMBL2315047 Kd = 15800.0 nM 4.80
CHEMBL2315047 Kd = 15848.93 nM 4.80