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Assay Detail

CHEMBL2320840

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of gamma secretase-mediated amyloid beta40 production in HEK293 cells transfected with human APPSOW and APPLON after 5 hrs by sandwich immunoassay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Gamma-secretase (CHEMBL2094135)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Structure activity relationship studies of tricyclic bispyran sulfone γ-secretase inhibitors.
Wu WL, Asberom T, Bara T, Bennett C, Burnett DA, Clader J, Domalski M, Greenlee WJ, Josien H, McBriar M, Rajagopalan M, Vicarel M, Xu R, Hyde LA, Del Vecchio RA, Cohen-Williams ME, Song L, Lee J, Terracina G, Zhang Q, Nomeir A, Parker EM, Zhang L.
Bioorg Med Chem Lett (2013) 23 : 844 -849
PubMed 23265892 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 7.99 8.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2314606 1 8.92
CHEMBL2314603 1 8.82
CHEMBL2314617 1 8.60
CHEMBL2159511 1 8.40
CHEMBL2159510 1 8.30
CHEMBL2159509 1 8.10
CHEMBL2314602 1 8.05
CHEMBL2314610 1 7.96
CHEMBL2314618 1 7.96
CHEMBL2314601 1 7.89
CHEMBL2314608 1 7.89
CHEMBL2314613 1 7.85
CHEMBL2314607 1 7.66
CHEMBL2314616 1 7.60
CHEMBL2314604 1 7.42
CHEMBL2314612 1 6.40
CHEMBL2314609 1 -
CHEMBL2314605 1 -
CHEMBL2314611 1 -
CHEMBL2314614 1 -
CHEMBL2314615 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2314606 IC50 = 1.2 nM 8.92
CHEMBL2314603 IC50 = 1.5 nM 8.82
CHEMBL2314617 IC50 = 2.5 nM 8.60
CHEMBL2159511 IC50 = 4.0 nM 8.40
CHEMBL2159510 IC50 = 5.0 nM 8.30
CHEMBL2159509 IC50 = 8.0 nM 8.10
CHEMBL2314602 IC50 = 8.9 nM 8.05
CHEMBL2314610 IC50 = 11.0 nM 7.96
CHEMBL2314618 IC50 = 11.0 nM 7.96
CHEMBL2314601 IC50 = 13.0 nM 7.89
CHEMBL2314608 IC50 = 13.0 nM 7.89
CHEMBL2314613 IC50 = 14.0 nM 7.85
CHEMBL2314607 IC50 = 22.0 nM 7.66
CHEMBL2314616 IC50 = 25.0 nM 7.60
CHEMBL2314604 IC50 = 38.0 nM 7.42
CHEMBL2314612 IC50 = 400.0 nM 6.40
CHEMBL2314609 IC50 - -
CHEMBL2314605 IC50 - -
CHEMBL2314611 IC50 - -
CHEMBL2314614 IC50 - -
CHEMBL2314615 IC50 - -