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Assay Detail

CHEMBL2327271

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of trypsin-like activity of rabbit 26s proteasome beta 2 subunit assessed as hydrolysis of Ac-RLR-amc fluorogenic substrate preincubated for 30 mins followed by substrate addition measured for 30 mins by fluorescence plate reader analysis
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Oryctolagus cuniculus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Incorporation of non-natural amino acids improves cell permeability and potency of specific inhibitors of proteasome trypsin-like sites.
Geurink PP, van der Linden WA, Mirabella AC, Gallastegui N, de Bruin G, Blom AE, Voges MJ, Mock ED, Florea BI, van der Marel GA, Driessen C, van der Stelt M, Groll M, Overkleeft HS, Kisselev AF.
J Med Chem (2013) 56 : 1262 -1275
PubMed 23320547 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 6.79 8.42

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2326533 1 8.42
CHEMBL2326262 1 8.20
CHEMBL2326261 1 8.18
CHEMBL2326541 1 7.92
CHEMBL2326260 1 7.52
CHEMBL2326263 1 7.35
CHEMBL2326539 1 7.23
CHEMBL2326271 1 7.10
CHEMBL2326535 1 6.96
CHEMBL2326264 1 6.75
CHEMBL2326534 1 6.60
CHEMBL2326272 1 6.52
CHEMBL2326537 1 6.38
CHEMBL2326538 1 6.13
CHEMBL2326270 1 6.00
CHEMBL2326536 1 5.75
CHEMBL2326265 1 4.62
CHEMBL2326266 1 4.62
CHEMBL2326540 1 -
CHEMBL2326267 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2326533 IC50 = 3.8 nM 8.42
CHEMBL2326262 IC50 = 6.3 nM 8.20
CHEMBL2326261 IC50 = 6.6 nM 8.18
CHEMBL2326541 IC50 = 12.0 nM 7.92
CHEMBL2326260 IC50 = 30.0 nM 7.52
CHEMBL2326263 IC50 = 45.0 nM 7.35
CHEMBL2326539 IC50 = 59.0 nM 7.23
CHEMBL2326271 IC50 = 80.0 nM 7.10
CHEMBL2326535 IC50 = 110.0 nM 6.96
CHEMBL2326264 IC50 = 180.0 nM 6.75
CHEMBL2326534 IC50 = 250.0 nM 6.60
CHEMBL2326272 IC50 = 300.0 nM 6.52
CHEMBL2326537 IC50 = 420.0 nM 6.38
CHEMBL2326538 IC50 = 740.0 nM 6.13
CHEMBL2326270 IC50 = 1000.0 nM 6.00
CHEMBL2326536 IC50 = 1800.0 nM 5.75
CHEMBL2326265 IC50 = 24000.0 nM 4.62
CHEMBL2326266 IC50 = 24000.0 nM 4.62
CHEMBL2326540 IC50 > 30000.0 nM -
CHEMBL2326267 IC50 > 30000.0 nM -