Assay Detail
Binding
CHEMBL2329030
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of JAK2/JAK1 in C57BL/6 mouse whole blood assessed as inhibition of IFN-gamma-stimulated STAT1 phosphorylation after 30 mins by flow cytometric analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Tissue | Blood |
| Confidence | 5 — Multiple protein complex / RNA |
| Curated By | Autocuration |
Target
Tyrosine-protein kinase JAK1/JAK2 (CHEMBL3885649) ↗| Type | PROTEIN FAMILY |
| Organism | Mus musculus |
Publication
3-Amido pyrrolopyrazine JAK kinase inhibitors: development of a JAK3 vs JAK1 selective inhibitor and evaluation in cellular and in vivo models.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 6.83 | 6.96 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2325898 | — | — | 1 | 6.96 |
| CHEMBL2325895 | — | — | 1 | 6.77 |
| CHEMBL221959 | TOFACITINIB | 4.0 | 1 | 6.75 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2325898 | — | IC50 | = | 110.0 | nM | 6.96 |
| CHEMBL2325895 | — | IC50 | = | 170.0 | nM | 6.77 |
| CHEMBL221959 | TOFACITINIB | IC50 | = | 180.0 | nM | 6.75 |