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Assay Detail

CHEMBL2329360

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of Clostridium botulinum truncated BoNT/A light chain (1-425) using truncated SNAP 25 (141-206) peptide as substrate by LC/MS analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Clostridium botulinum
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Botulinum neurotoxin type A (CHEMBL5192)
Type SINGLE PROTEIN
Organism Clostridium botulinum

Publication

The C-terminus of Botulinum A Protease Has Profound and Unanticipated Kinetic Consequences Upon the Catalytic Cleft.
Silhár P, Lardy MA, Hixon MS, Shoemaker CB, Barbieri JT, Struss AK, Lively JM, Javor S, Janda KD.
ACS Med Chem Lett (2013) 4 : 283 -287
PubMed 23565325 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 5.62 6.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL237370 CINNAMIC ACID HYDROXAMATE 1 6.52
CHEMBL29660 CHICORIC ACID 1 5.17
CHEMBL1505471 LOMOFUNGIN -1.0 1 5.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL237370 CINNAMIC ACID HYDROXAMATE Ki = 300.0 nM 6.52
CHEMBL29660 CHICORIC ACID Ki = 6700.0 nM 5.17
CHEMBL1505471 LOMOFUNGIN Ki = 6700.0 nM 5.17