Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL2329569

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of JAK1 (unknown origin)-mediated phosphorylation of Biotin-KAIETDKEYYTVKD incubated for 10 mins prior to substrate addition measured after 1 hr by scintillation counting analysis in presence of [gamma-33P]ATP
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK1 (CHEMBL2835)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

3-Amido pyrrolopyrazine JAK kinase inhibitors: development of a JAK3 vs JAK1 selective inhibitor and evaluation in cellular and in vivo models.
Soth M, Hermann JC, Yee C, Alam M, Barnett JW, Berry P, Browner MF, Frank K, Frauchiger S, Harris S, He Y, Hekmat-Nejad M, Hendricks T, Henningsen R, Hilgenkamp R, Ho H, Hoffman A, Hsu PY, Hu DQ, Itano A, Jaime-Figueroa S, Jahangir A, Jin S, Kuglstatter A, Kutach AK, Liao C, Lynch S, Menke J, Niu L, Patel V, Railkar A, Roy D, Shao A, Shaw D, Steiner S, Sun Y, Tan SL, Wang S, Vu MD.
J Med Chem (2013) 56 : 345 -356
PubMed 23214979 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 7.24 9.33

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2325897 1 9.33
CHEMBL2325898 1 9.23
CHEMBL221959 TOFACITINIB 4.0 1 8.80
CHEMBL2325895 1 8.49
CHEMBL2325900 1 7.62
CHEMBL2325912 1 7.35
CHEMBL2325899 1 7.35
CHEMBL2325903 1 7.35
CHEMBL2325894 1 7.33
CHEMBL2325902 1 7.12
CHEMBL2325905 1 7.00
CHEMBL2325906 1 6.97
CHEMBL2325901 1 6.65
CHEMBL2325904 1 6.59
CHEMBL2325913 1 6.57
CHEMBL2325909 1 6.44
CHEMBL2325910 1 6.37
CHEMBL2325908 1 6.20
CHEMBL2325911 1 6.06
CHEMBL2325907 1 5.96
CHEMBL2325896 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2325897 IC50 = 0.47 nM 9.33
CHEMBL2325898 IC50 = 0.59 nM 9.23
CHEMBL221959 TOFACITINIB IC50 = 1.6 nM 8.80
CHEMBL2325895 IC50 = 3.2 nM 8.49
CHEMBL2325900 IC50 = 24.0 nM 7.62
CHEMBL2325912 IC50 = 44.7 nM 7.35
CHEMBL2325899 IC50 = 44.8 nM 7.35
CHEMBL2325903 IC50 = 44.5 nM 7.35
CHEMBL2325894 IC50 = 47.0 nM 7.33
CHEMBL2325902 IC50 = 75.8 nM 7.12
CHEMBL2325905 IC50 = 99.7 nM 7.00
CHEMBL2325906 IC50 = 108.1 nM 6.97
CHEMBL2325901 IC50 = 224.3 nM 6.65
CHEMBL2325904 IC50 = 256.7 nM 6.59
CHEMBL2325913 IC50 = 272.0 nM 6.57
CHEMBL2325909 IC50 = 361.0 nM 6.44
CHEMBL2325910 IC50 = 430.5 nM 6.37
CHEMBL2325908 IC50 = 636.6 nM 6.20
CHEMBL2325911 IC50 = 870.7 nM 6.06
CHEMBL2325907 IC50 = 1102.5 nM 5.96
CHEMBL2325896 IC50 < 0.5 nM -