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Assay Detail

CHEMBL2329661

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human SGLT2 expressed in COS7 cells assessed as inhibition [14C]-AMG cellular uptake
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type COS-7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium/glucose cotransporter 2 (CHEMBL3884)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and structure-activity relationships of a series of 4-benzyl-5-isopropyl-1H-pyrazol-3-yl β-D-glycopyranosides substituted with novel hydrophilic groups as highly potent inhibitors of sodium glucose co-transporter 1 (SGLT1).
Fushimi N, Teranishi H, Shimizu K, Yonekubo S, Ohno K, Miyagi T, Itoh F, Shibazaki T, Tomae M, Ishikawa-Takemura Y, Nakabayashi T, Kamada N, Yamauchi Y, Kobayashi S, Isaji M.
Bioorg Med Chem (2013) 21 : 748 -765
PubMed 23273606 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 6.30 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2323692 1 8.10
CHEMBL2323684 1 7.75
CHEMBL2323683 1 7.70
CHEMBL2323405 1 7.38
CHEMBL2323409 1 7.34
CHEMBL2323693 1 7.10
CHEMBL2323406 1 7.01
CHEMBL2323407 1 6.82
CHEMBL2323408 1 6.68
CHEMBL2159106 1 6.54
CHEMBL2323417 1 6.50
CHEMBL2323416 1 6.45
CHEMBL2323695 1 6.43
CHEMBL2323414 1 6.34
CHEMBL2323415 1 6.22
CHEMBL2323689 1 6.03
CHEMBL2323418 1 5.98
CHEMBL2323413 1 5.97
CHEMBL2323685 1 5.80
CHEMBL2323694 1 5.76
CHEMBL2160193 1 5.70
CHEMBL2323410 1 5.61
CHEMBL2323412 1 5.59
CHEMBL2323686 1 5.51
CHEMBL2323688 1 5.40
CHEMBL2323690 1 5.33
CHEMBL2323687 1 5.29
CHEMBL2323691 1 5.25
CHEMBL2323411 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2323692 IC50 = 8.0 nM 8.10
CHEMBL2323684 IC50 = 18.0 nM 7.75
CHEMBL2323683 IC50 = 20.0 nM 7.70
CHEMBL2323405 IC50 = 42.0 nM 7.38
CHEMBL2323409 IC50 = 46.0 nM 7.34
CHEMBL2323693 IC50 = 80.0 nM 7.10
CHEMBL2323406 IC50 = 98.0 nM 7.01
CHEMBL2323407 IC50 = 151.0 nM 6.82
CHEMBL2323408 IC50 = 209.0 nM 6.68
CHEMBL2159106 IC50 = 285.0 nM 6.54
CHEMBL2323417 IC50 = 319.0 nM 6.50
CHEMBL2323416 IC50 = 358.0 nM 6.45
CHEMBL2323695 IC50 = 371.0 nM 6.43
CHEMBL2323414 IC50 = 457.0 nM 6.34
CHEMBL2323415 IC50 = 608.0 nM 6.22
CHEMBL2323689 IC50 = 936.0 nM 6.03
CHEMBL2323418 IC50 = 1050.0 nM 5.98
CHEMBL2323413 IC50 = 1070.0 nM 5.97
CHEMBL2323685 IC50 = 1590.0 nM 5.80
CHEMBL2323694 IC50 = 1730.0 nM 5.76
CHEMBL2160193 IC50 = 2020.0 nM 5.70
CHEMBL2323410 IC50 = 2470.0 nM 5.61
CHEMBL2323412 IC50 = 2540.0 nM 5.59
CHEMBL2323686 IC50 = 3090.0 nM 5.51
CHEMBL2323688 IC50 = 3980.0 nM 5.40
CHEMBL2323690 IC50 = 4700.0 nM 5.33
CHEMBL2323687 IC50 = 5100.0 nM 5.29
CHEMBL2323691 IC50 = 5620.0 nM 5.25
CHEMBL2323411 IC50 = 9950.0 nM 5.00