Assay Detail
Binding
CHEMBL2330526
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human recombinant N-terminus 6X-histidine-tagged indoleamine 2,3-dioxygenase expressed in Escherichia coli assessed as inhibition of L-tryptophan to N-formylkynurenine formation measured as residual enzyme activity incubated for 15 mins prior to substrate addition measured after 30 mins by UV-vis spectrophotometric analysis relative to DMSO-treated control
3
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Aminophenoxazinones as inhibitors of indoleamine 2,3-dioxygenase (IDO). Synthesis of exfoliazone and chandrananimycin A.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 5.25 | 6.34 |
| Ki | 1 | 6.49 | 6.49 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2322655 | CINNABARINIC ACID | — | 2 | 6.49 |
| CHEMBL14145 | — | — | 1 | 4.15 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2322655 | CINNABARINIC ACID | Ki | = | 326.0 | nM | 6.49 |
| CHEMBL2322655 | CINNABARINIC ACID | IC50 | = | 460.0 | nM | 6.34 |
| CHEMBL14145 | — | IC50 | = | 70300.0 | nM | 4.15 |