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Assay Detail

CHEMBL2330631

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ATR-mediated CHK1 phosphorylation at serine 345 in human HT29 cells after 1 hr in presence of 4-nitroquinoline 1-oxide
38
Total Activities
38
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HT-29
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase ATR (CHEMBL5024)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 4-{4-[(3R)-3-Methylmorpholin-4-yl]-6-[1-(methylsulfonyl)cyclopropyl]pyrimidin-2-yl}-1H-indole (AZ20): a potent and selective inhibitor of ATR protein kinase with monotherapy in vivo antitumor activity.
Foote KM, Blades K, Cronin A, Fillery S, Guichard SS, Hassall L, Hickson I, Jacq X, Jewsbury PJ, McGuire TM, Nissink JW, Odedra R, Page K, Perkins P, Suleman A, Tam K, Thommes P, Broadhurst R, Wood C.
J Med Chem (2013) 56 : 2125 -2138
PubMed 23394205 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 38 5.76 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2325697 1 7.30
CHEMBL2325705 1 6.75
CHEMBL2325703 1 6.55
CHEMBL2325699 1 6.46
CHEMBL2325704 1 6.44
CHEMBL2325706 1 6.38
CHEMBL2325439 1 6.36
CHEMBL2325720 1 6.31
CHEMBL2325711 1 6.23
CHEMBL2325440 1 6.14
CHEMBL2325708 1 6.11
CHEMBL2325695 1 6.10
CHEMBL2030442 1 5.96
CHEMBL2325724 1 5.82
CHEMBL2325712 1 5.70
CHEMBL2325718 1 5.60
CHEMBL2325709 1 5.55
CHEMBL2325710 1 5.54
CHEMBL2325707 1 5.43
CHEMBL2325696 1 5.43
CHEMBL2325698 1 5.36
CHEMBL2325721 1 5.20
CHEMBL2325723 1 5.14
CHEMBL2325717 1 5.05
CHEMBL2325438 1 5.04
CHEMBL2325716 1 4.89
CHEMBL2325726 1 4.82
CHEMBL2325715 1 4.80
CHEMBL2325725 1 4.62
CHEMBL2325722 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2325697 IC50 = 50.0 nM 7.30
CHEMBL2325705 IC50 = 180.0 nM 6.75
CHEMBL2325703 IC50 = 280.0 nM 6.55
CHEMBL2325699 IC50 = 350.0 nM 6.46
CHEMBL2325704 IC50 = 360.0 nM 6.44
CHEMBL2325706 IC50 = 420.0 nM 6.38
CHEMBL2325439 IC50 = 440.0 nM 6.36
CHEMBL2325720 IC50 = 490.0 nM 6.31
CHEMBL2325711 IC50 = 590.0 nM 6.23
CHEMBL2325440 IC50 = 720.0 nM 6.14
CHEMBL2325708 IC50 = 770.0 nM 6.11
CHEMBL2325695 IC50 = 800.0 nM 6.10
CHEMBL2030442 IC50 = 1100.0 nM 5.96
CHEMBL2325724 IC50 = 1500.0 nM 5.82
CHEMBL2325712 IC50 = 2000.0 nM 5.70
CHEMBL2325718 IC50 = 2500.0 nM 5.60
CHEMBL2325709 IC50 = 2800.0 nM 5.55
CHEMBL2325710 IC50 = 2900.0 nM 5.54
CHEMBL2325696 IC50 = 3700.0 nM 5.43
CHEMBL2325707 IC50 = 3700.0 nM 5.43
CHEMBL2325698 IC50 = 4400.0 nM 5.36
CHEMBL2325721 IC50 = 6300.0 nM 5.20
CHEMBL2325723 IC50 = 7200.0 nM 5.14
CHEMBL2325717 IC50 = 8900.0 nM 5.05
CHEMBL2325438 IC50 = 9200.0 nM 5.04
CHEMBL2325716 IC50 = 13000.0 nM 4.89
CHEMBL2325726 IC50 = 15000.0 nM 4.82
CHEMBL2325715 IC50 = 16000.0 nM 4.80
CHEMBL2325725 IC50 = 24000.0 nM 4.62
CHEMBL2325722 IC50 > 30000.0 nM -
CHEMBL2030436 IC50 > 30000.0 nM -
CHEMBL2325700 IC50 > 30000.0 nM -
CHEMBL2325701 IC50 > 30000.0 nM -
CHEMBL2325702 IC50 > 30000.0 nM -
CHEMBL2325714 IC50 > 30000.0 nM -
CHEMBL2325719 IC50 > 30000.0 nM -
CHEMBL2321916 IC50 > 30000.0 nM -
CHEMBL2325713 IC50 > 30000.0 nM -