Assay Detail
Binding
CHEMBL2339337
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Escherichia coli MurA expressed in Escherichia coli BL21(lambdaDE3) using UNAG and PEP as substrate incubated for 10 mins prior to PEP addition measured after 60 mins by spectrophotometric analysis
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
UDP-N-acetylglucosamine 1-carboxyvinyltransferase (CHEMBL1984) ↗| Type | SINGLE PROTEIN |
| Organism | Escherichia coli K-12 |
Publication
Chemical, biochemical and microbiological properties of a brominated nitrovinylfuran with broad-spectrum antibacterial activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 7 | 5.45 | 7.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1757 | FOSFOMYCIN | 4.0 | 1 | 7.00 |
| CHEMBL2335230 | — | — | 1 | 5.55 |
| CHEMBL2335229 | BROMO(NITRO)METHANE | — | 1 | 5.34 |
| CHEMBL1257707 | CNICIN | — | 1 | 4.78 |
| CHEMBL2335231 | — | — | 1 | 4.56 |
| CHEMBL276924 | NITROMETHANE | — | 1 | - |
| CHEMBL2335228 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1757 | FOSFOMYCIN | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL2335230 | — | IC50 | = | 2800.0 | nM | 5.55 |
| CHEMBL2335229 | BROMO(NITRO)METHANE | IC50 | = | 4600.0 | nM | 5.34 |
| CHEMBL1257707 | CNICIN | IC50 | = | 16700.0 | nM | 4.78 |
| CHEMBL2335231 | — | IC50 | = | 27300.0 | nM | 4.56 |
| CHEMBL276924 | NITROMETHANE | IC50 | - | — | - | |
| CHEMBL2335228 | — | IC50 | - | — | - |