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Assay Detail

CHEMBL2343053

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human 5HT2C receptor expressed in HEK293 cells assessed as inhibition of 5HT-induced intracellular calcium release measured for 90 secs by fluorescence assay
8
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2C (CHEMBL225)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and pharmacological characterization of N- and O-substituted 5,6,7,8-tetrahydro-4H-isoxazolo[4,5-d]azepin-3-ol analogues: novel 5-HT(2A)/5-HT(2C) receptor agonists with pro-cognitive properties.
Jensen AA, Plath N, Pedersen MH, Isberg V, Krall J, Wellendorph P, Stensbøl TB, Gloriam DE, Krogsgaard-Larsen P, Frølund B.
J Med Chem (2013) 56 : 1211 -1227
PubMed 23301527 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 7.21 8.61

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3187365 ASENAPINE 4.0 2 8.61
CHEMBL6437 MIANSERIN 4.0 2 8.10
CHEMBL42 CLOZAPINE 4.0 2 7.14
CHEMBL18972 GLEMANSERIN 2.0 2 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3187365 ASENAPINE Ki = 2.455 nM 8.61
CHEMBL3187365 ASENAPINE Ki = 2.5 nM 8.60
CHEMBL6437 MIANSERIN Ki = 8.0 nM 8.10
CHEMBL6437 MIANSERIN Ki = 8.128 nM 8.09
CHEMBL42 CLOZAPINE Ki = 72.44 nM 7.14
CHEMBL42 CLOZAPINE Ki = 73.0 nM 7.14
CHEMBL18972 GLEMANSERIN Ki = 10000.0 nM 5.00
CHEMBL18972 GLEMANSERIN Ki = 10000.0 nM 5.00