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Assay Detail

CHEMBL2344115

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant NEK1 (unknown origin) by FRET-based Z'-LYTE assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase Nek1 (CHEMBL5855)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Monocarbonyl curcumin analogues: heterocyclic pleiotropic kinase inhibitors that mediate anticancer properties.
Brown A, Shi Q, Moore TW, Yoon Y, Prussia A, Maddox C, Liotta DC, Shim H, Snyder JP.
J Med Chem (2013) 56 : 3456 -3466
PubMed 23550937 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 4.81 6.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL574646 1 6.30
CHEMBL2338333 1 5.46
CHEMBL2036343 1 4.85
CHEMBL379849 1 4.11
CHEMBL2338335 1 4.08
CHEMBL2338334 1 4.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL574646 IC50 = 500.0 nM 6.30
CHEMBL2338333 IC50 = 3500.0 nM 5.46
CHEMBL2036343 IC50 = 14000.0 nM 4.85
CHEMBL379849 IC50 = 77000.0 nM 4.11
CHEMBL2338335 IC50 = 83000.0 nM 4.08
CHEMBL2338334 IC50 = 83000.0 nM 4.08