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Assay Detail

CHEMBL2345514

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Mixed type inhibition of chymotrypsin-like activity of human constitutive 20s proteasome beta-5 subunit using Suc-LLVY-AMC as substrate assessed as inhibition constant for dissociation of enzyme-substrate-inhibitor complex incubated for 15 mins prior to substrate addition measured after 45 mins by fluorescence assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proteasome Macropain subunit MB1 (CHEMBL4662)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Dimerized linear mimics of a natural cyclopeptide (TMC-95A) are potent noncovalent inhibitors of the eukaryotic 20S proteasome.
Desvergne A, Genin E, Maréchal X, Gallastegui N, Dufau L, Richy N, Groll M, Vidal J, Reboud-Ravaux M.
J Med Chem (2013) 56 : 3367 -3378
PubMed 23540790 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 6.17 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2337842 1 6.70
CHEMBL2337841 1 6.50
CHEMBL2337849 1 5.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2337842 Ki = 200.0 nM 6.70
CHEMBL2337841 Ki = 316.0 nM 6.50
CHEMBL2337849 Ki = 5030.0 nM 5.30