Assay Detail
Binding
CHEMBL2345533
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to his-tagged Mcl-1 R263A mutant (unknown origin) expressed in Escherichia coli BL21 (DE3) cells by ITC analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Leukemia cell |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Induced myeloid leukemia cell differentiation protein Mcl-1 (CHEMBL4361) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Novel soluble myeloid cell leukemia sequence 1 (Mcl-1) inhibitor (E,E)-2-(benzylaminocarbonyl)-3-styrylacrylonitrile (4g) developed using a fragment-based approach.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 5 | 4.46 | 5.17 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2333148 | — | — | 1 | 5.17 |
| CHEMBL2333143 | — | — | 1 | 4.34 |
| CHEMBL2314369 | — | — | 1 | 4.29 |
| CHEMBL2333147 | — | — | 1 | 4.04 |
| CHEMBL2333142 | CYANOACETAMIDE | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2333148 | — | Kd | = | 6720.0 | nM | 5.17 |
| CHEMBL2333143 | — | Kd | = | 46170.0 | nM | 4.34 |
| CHEMBL2314369 | — | Kd | = | 51390.0 | nM | 4.29 |
| CHEMBL2333147 | — | Kd | = | 90830.0 | nM | 4.04 |
| CHEMBL2333142 | CYANOACETAMIDE | Kd | > | 1000000.0 | nM | - |