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Assay Detail

CHEMBL2345601

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant MMP14 catalytic domain using Mca-Lys-Pro- Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 as substrate incubated for 2 hrs prior to substrate addition measured every 15 secs for 15 mins by fluorometric analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Matrix metalloproteinase-14 (CHEMBL3869)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Arylsulfonamide inhibitors of aggrecanases as potential therapeutic agents for osteoarthritis: synthesis and biological evaluation.
Nuti E, Santamaria S, Casalini F, Yamamoto K, Marinelli L, La Pietra V, Novellino E, Orlandini E, Nencetti S, Marini AM, Salerno S, Taliani S, Da Settimo F, Nagase H, Rossello A.
Eur J Med Chem (2013) 62 : 379 -394
PubMed 23376997 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 7.18 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL198778 1 8.40
CHEMBL19611 ILOMASTAT 2.0 1 8.33
CHEMBL2337697 1 7.62
CHEMBL197842 1 7.15
CHEMBL2337690 1 7.09
CHEMBL2337696 1 6.87
CHEMBL2337691 1 6.51
CHEMBL2337698 1 5.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL198778 IC50 = 4.0 nM 8.40
CHEMBL19611 ILOMASTAT IC50 = 4.7 nM 8.33
CHEMBL2337697 IC50 = 24.0 nM 7.62
CHEMBL197842 IC50 = 71.0 nM 7.15
CHEMBL2337690 IC50 = 82.0 nM 7.09
CHEMBL2337696 IC50 = 135.0 nM 6.87
CHEMBL2337691 IC50 = 310.0 nM 6.51
CHEMBL2337698 IC50 = 3700.0 nM 5.43