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Assay Detail

CHEMBL2345645

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of goat brain tubulin polymerization incubated for 20 mins prior to GTP addition measured for 30 mins by 90 degree light scattering-based fluorescence spectrophotometric method
6
Total Activities
5
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Capra hircus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

A synthetic dolastatin 10 analogue suppresses microtubule dynamics, inhibits cell proliferation, and induces apoptotic cell death.
Gajula PK, Asthana J, Panda D, Chakraborty TK.
J Med Chem (2013) 56 : 2235 -2245
PubMed 23445405 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.35 5.92
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL39541 DOLASTATIN-10 2.0 1 5.92
CHEMBL159 VINBLASTINE 4.0 1 5.82
CHEMBL2332012 2 5.22
CHEMBL379989 RHIZOXIN 1 5.17
CHEMBL35769 DOLASTATIN 15 1 4.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL39541 DOLASTATIN-10 IC50 = 1200.0 nM 5.92
CHEMBL159 VINBLASTINE IC50 = 1500.0 nM 5.82
CHEMBL2332012 IC50 = 6000.0 nM 5.22
CHEMBL379989 RHIZOXIN IC50 = 6800.0 nM 5.17
CHEMBL35769 DOLASTATIN 15 IC50 = 23000.0 nM 4.64
CHEMBL2332012 Inhibition - % -