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Assay Detail

CHEMBL2345965

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of postacid activity of human constitutive 20s proteasome beta-1 subunit using Z-LLE-betaNA as substrate assessed as remaining activity incubated for 15 mins prior to substrate addition measured after 45 mins by fluorescence assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proteasome component C5 (CHEMBL4208)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Dimerized linear mimics of a natural cyclopeptide (TMC-95A) are potent noncovalent inhibitors of the eukaryotic 20S proteasome.
Desvergne A, Genin E, Maréchal X, Gallastegui N, Dufau L, Richy N, Groll M, Vidal J, Reboud-Ravaux M.
J Med Chem (2013) 56 : 3367 -3378
PubMed 23540790 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 5.93 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2337848 1 7.22
CHEMBL2337847 1 6.47
CHEMBL2337846 1 6.46
CHEMBL2337844 1 6.43
CHEMBL2337845 1 6.35
CHEMBL2337843 1 6.23
CHEMBL2337842 1 5.59
CHEMBL2337841 1 5.34
CHEMBL2337849 1 4.94
CHEMBL2337850 1 4.29
CHEMBL2337440 1 -
CHEMBL2337439 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2337848 IC50 = 60.0 nM 7.22
CHEMBL2337847 IC50 = 336.0 nM 6.47
CHEMBL2337846 IC50 = 346.0 nM 6.46
CHEMBL2337844 IC50 = 370.0 nM 6.43
CHEMBL2337845 IC50 = 448.0 nM 6.35
CHEMBL2337843 IC50 = 590.0 nM 6.23
CHEMBL2337842 IC50 = 2560.0 nM 5.59
CHEMBL2337841 IC50 = 4577.0 nM 5.34
CHEMBL2337849 IC50 = 11520.0 nM 4.94
CHEMBL2337850 IC50 = 51200.0 nM 4.29
CHEMBL2337440 IC50 - -
CHEMBL2337439 IC50 - -