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Assay Detail

CHEMBL2352972

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant Toxoplasma gondii DXR catalytic domain (68 to 513) expressed in Escherichia coli BL21 using DXP as substrate after 10 mins by Uv-spectrophotometric analysis
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Toxoplasma gondii
Tissue Plasma
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Expression, characterization and inhibition of Toxoplasma gondii 1-deoxy-D-xylulose-5-phosphate reductoisomerase.
Cai G, Deng L, Xue J, Moreno SN, Striepen B, Song Y.
Bioorg Med Chem Lett (2013) 23 : 2158 -2161
PubMed 23428849 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 6.40 7.32

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL205338 FR-900098 1 7.32
CHEMBL2323928 1 7.26
CHEMBL204106 1 7.11
CHEMBL2323929 1 7.10
CHEMBL203125 FOSMIDOMYCIN 3.0 1 7.05
CHEMBL204107 1 6.66
CHEMBL2323931 1 6.28
CHEMBL2323930 1 6.01
CHEMBL1615246 1 5.68
CHEMBL1615245 PYRIDIN-2-YLMETHYLPHOSPHONIC ACID 1 5.39
CHEMBL574580 1-HYDROXY-5-PHENYLPYRIDIN-2(1H)-ONE 1 4.59

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL205338 FR-900098 Ki = 48.0 nM 7.32
CHEMBL2323928 Ki = 55.0 nM 7.26
CHEMBL204106 Ki = 77.0 nM 7.11
CHEMBL2323929 Ki = 79.0 nM 7.10
CHEMBL203125 FOSMIDOMYCIN Ki = 90.0 nM 7.05
CHEMBL204107 Ki = 220.0 nM 6.66
CHEMBL2323931 Ki = 530.0 nM 6.28
CHEMBL2323930 Ki = 970.0 nM 6.01
CHEMBL1615246 Ki = 2100.0 nM 5.68
CHEMBL1615245 PYRIDIN-2-YLMETHYLPHOSPHONIC ACID Ki = 4100.0 nM 5.39
CHEMBL574580 1-HYDROXY-5-PHENYLPYRIDIN-2(1H)-ONE Ki = 25600.0 nM 4.59