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Assay Detail

CHEMBL2353035

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Functional
Agonist activity at human kappa opioid receptor expressed in CHO cell membrane assessed as stimulation of [35S]GTPgammaS binding after 60 mins by liquid scintillation counting
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kappa-type opioid receptor (CHEMBL237)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Redefining the structure-activity relationships of 2,6-methano-3-benzazocines. Part 9: Synthesis, characterization and molecular modeling of pyridinyl isosteres of N-BPE-8-CAC (1), a high affinity ligand for opioid receptors.
VanAlstine MA, Wentland MP, Alvarez J, Cao Q, Cohen DJ, Knapp BI, Bidlack JM.
Bioorg Med Chem Lett (2013) 23 : 2128 -2133
PubMed 23434225 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 8 8.11 8.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2347237 1 8.59
CHEMBL377789 1 8.52
CHEMBL58646 1 8.36
CHEMBL2347235 1 8.30
CHEMBL2347238 1 8.11
CHEMBL2347239 1 7.85
CHEMBL2347236 1 7.72
CHEMBL290543 1 7.44

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2347237 EC50 = 2.6 nM 8.59
CHEMBL377789 EC50 = 3.0 nM 8.52
CHEMBL58646 EC50 = 4.4 nM 8.36
CHEMBL2347235 EC50 = 5.0 nM 8.30
CHEMBL2347238 EC50 = 7.8 nM 8.11
CHEMBL2347239 EC50 = 14.0 nM 7.85
CHEMBL2347236 EC50 = 19.0 nM 7.72
CHEMBL290543 EC50 = 36.0 nM 7.44