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Assay Detail

CHEMBL2353183

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human BACE1 using MR121-labeled substrate incubated for 4 mins prior to substrate addition measured after 2 mins by spectrophotometric analysis
37
Total Activities
37
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-secretase 1 (CHEMBL4822)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

β-Secretase (BACE1) inhibitors with high in vivo efficacy suitable for clinical evaluation in Alzheimer's disease.
Hilpert H, Guba W, Woltering TJ, Wostl W, Pinard E, Mauser H, Mayweg AV, Rogers-Evans M, Humm R, Krummenacher D, Muser T, Schnider C, Jacobsen H, Ozmen L, Bergadano A, Banner DW, Hochstrasser R, Kuglstatter A, David-Pierson P, Fischer H, Polara A, Narquizian R.
J Med Chem (2013) 56 : 3980 -3995
PubMed 23590342 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 37 7.24 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2347198 1 8.22
CHEMBL2347208 1 7.92
CHEMBL2347188 1 7.89
CHEMBL2347197 1 7.89
CHEMBL2347212 1 7.85
CHEMBL2347211 1 7.80
CHEMBL2347192 1 7.77
CHEMBL2347187 1 7.72
CHEMBL2347202 1 7.72
CHEMBL2347215 1 7.64
CHEMBL2347196 1 7.60
CHEMBL2347210 1 7.58
CHEMBL2347193 1 7.58
CHEMBL2347195 1 7.57
CHEMBL2347204 1 7.55
CHEMBL2347364 1 7.50
CHEMBL2347201 1 7.44
CHEMBL2347189 1 7.40
CHEMBL2347191 1 7.38
CHEMBL2347190 1 7.31
CHEMBL2347203 1 7.29
CHEMBL2347365 1 7.29
CHEMBL2347206 1 7.27
CHEMBL2347366 1 7.24
CHEMBL2347214 1 7.18
CHEMBL2347194 1 7.17
CHEMBL2347186 1 7.11
CHEMBL2347363 1 7.06
CHEMBL2347368 1 6.97
CHEMBL2347209 1 6.86

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2347198 IC50 = 6.0 nM 8.22
CHEMBL2347208 IC50 = 12.0 nM 7.92
CHEMBL2347197 IC50 = 13.0 nM 7.89
CHEMBL2347188 IC50 = 13.0 nM 7.89
CHEMBL2347212 IC50 = 14.0 nM 7.85
CHEMBL2347211 IC50 = 16.0 nM 7.80
CHEMBL2347192 IC50 = 17.0 nM 7.77
CHEMBL2347187 IC50 = 19.0 nM 7.72
CHEMBL2347202 IC50 = 19.0 nM 7.72
CHEMBL2347215 IC50 = 23.0 nM 7.64
CHEMBL2347196 IC50 = 25.0 nM 7.60
CHEMBL2347210 IC50 = 26.0 nM 7.58
CHEMBL2347193 IC50 = 26.0 nM 7.58
CHEMBL2347195 IC50 = 27.0 nM 7.57
CHEMBL2347204 IC50 = 28.0 nM 7.55
CHEMBL2347364 IC50 = 32.0 nM 7.50
CHEMBL2347201 IC50 = 36.0 nM 7.44
CHEMBL2347189 IC50 = 40.0 nM 7.40
CHEMBL2347191 IC50 = 42.0 nM 7.38
CHEMBL2347190 IC50 = 49.0 nM 7.31
CHEMBL2347203 IC50 = 51.0 nM 7.29
CHEMBL2347365 IC50 = 51.0 nM 7.29
CHEMBL2347206 IC50 = 54.0 nM 7.27
CHEMBL2347366 IC50 = 57.0 nM 7.24
CHEMBL2347214 IC50 = 66.0 nM 7.18
CHEMBL2347194 IC50 = 67.0 nM 7.17
CHEMBL2347186 IC50 = 77.0 nM 7.11
CHEMBL2347363 IC50 = 87.0 nM 7.06
CHEMBL2347368 IC50 = 108.0 nM 6.97
CHEMBL2347209 IC50 = 137.0 nM 6.86
CHEMBL2347200 IC50 = 151.0 nM 6.82
CHEMBL2347367 IC50 = 184.0 nM 6.74
CHEMBL2347213 IC50 = 294.0 nM 6.53
CHEMBL2347205 IC50 = 435.0 nM 6.36
CHEMBL2347199 IC50 = 446.0 nM 6.35
CHEMBL2347207 IC50 = 776.0 nM 6.11
CHEMBL2347369 IC50 = 41200.0 nM 4.38