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Assay Detail

CHEMBL2379295

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Functional
Antimitotic activity in human HeLa cells assessed as cell density loss after 24 hrs by Hoechst 33342 staining
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HeLa
Confidence 1 — Organism
Curated By Autocuration

Target

HeLa (CHEMBL399)
Type CELL-LINE
Organism Homo sapiens

Publication

Cytotoxic 3,4,5-trimethoxychalcones as mitotic arresters and cell migration inhibitors.
Salum LB, Altei WF, Chiaradia LD, Cordeiro MN, Canevarolo RR, Melo CP, Winter E, Mattei B, Daghestani HN, Santos-Silva MC, Creczynski-Pasa TB, Yunes RA, Yunes JA, Andricopulo AD, Day BW, Nunes RJ, Vogt A.
Eur J Med Chem (2013) 63 : 501 -510
PubMed 23524161 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 6.40 7.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL107 COLCHICINE 4.0 1 7.49
CHEMBL285901 1 6.45
CHEMBL220818 1 6.17
CHEMBL484145 1 5.48
CHEMBL1946988 1 -
CHEMBL7976 CHALCONE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL107 COLCHICINE EC50 = 32.5 nM 7.49
CHEMBL285901 EC50 = 357.0 nM 6.45
CHEMBL220818 EC50 = 684.0 nM 6.17
CHEMBL484145 EC50 = 3300.0 nM 5.48
CHEMBL1946988 EC50 > 50000.0 nM -
CHEMBL7976 CHALCONE EC50 > 50000.0 nM -