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Assay Detail

CHEMBL2379695

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Binding
Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H4 receptor (CHEMBL3759)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A novel series of histamine H4 receptor antagonists based on the pyrido[3,2-d]pyrimidine scaffold: comparison of hERG binding and target residence time with PF-3893787.
Andaloussi M, Lim HD, van der Meer T, Sijm M, Poulie CB, de Esch IJ, Leurs R, Smits RA.
Bioorg Med Chem Lett (2013) 23 : 2663 -2670
PubMed 23558237 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 7 7.86 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL129198 JNJ-7777120 1 8.40
CHEMBL2376800 1 8.07
CHEMBL2376803 1 7.99
CHEMBL2376804 1 7.87
CHEMBL2376801 1 7.67
CHEMBL1915540 ADRIFORANT 2.0 1 7.60
CHEMBL1935574 1 7.39

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL129198 JNJ-7777120 Kd = 3.981 nM 8.40
CHEMBL2376800 Kd = 8.511 nM 8.07
CHEMBL2376803 Kd = 10.23 nM 7.99
CHEMBL2376804 Kd = 13.49 nM 7.87
CHEMBL2376801 Kd = 21.38 nM 7.67
CHEMBL1915540 ADRIFORANT Kd = 25.12 nM 7.60
CHEMBL1935574 Kd = 40.74 nM 7.39