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Assay Detail

CHEMBL2383017

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-fused human recombinant PI3Kdelta expressed in baculovirus infected SF9 cells after 1 hr by scintillation proximity assay in presence of [gamma-33P]-ATP
18
Total Activities
18
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of thiazolobenzoxepin PI3-kinase inhibitors that spare the PI3-kinase β isoform.
Staben ST, Ndubaku C, Blaquiere N, Belvin M, Bull RJ, Dudley D, Edgar K, Gray D, Heald R, Heffron TP, Jones GE, Jones M, Kolesnikov A, Lee L, Lesnick J, Lewis C, Murray J, McLean NJ, Nonomiya J, Olivero AG, Ord R, Pang J, Price S, Prior WW, Rouge L, Salphati L, Sampath D, Wallin J, Wang L, Wei B, Weismann C, Wu P.
Bioorg Med Chem Lett (2013) 23 : 2606 -2613
PubMed 23540645 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 8.51 9.22
Ki 2 8.36 9.21

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2381271 1 9.22
CHEMBL2381373 1 9.21
CHEMBL2381266 1 8.96
CHEMBL2381268 1 8.89
CHEMBL2381273 1 8.85
CHEMBL2381270 1 8.59
CHEMBL2381267 1 8.48
CHEMBL2381261 1 8.47
CHEMBL2381253 1 8.46
CHEMBL2322764 1 8.41
CHEMBL2381272 1 8.12
CHEMBL2381262 1 8.09
CHEMBL2381264 1 8.08
CHEMBL2381263 1 7.99
CHEMBL521851 PICTILISIB 2.0 1 7.51
CHEMBL2381269 1 -
CHEMBL2381265 1 -
CHEMBL2381379 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2381271 IC50 = 0.6 nM 9.22
CHEMBL2381373 Ki = 0.61 nM 9.21
CHEMBL2381266 IC50 = 1.1 nM 8.96
CHEMBL2381268 IC50 = 1.3 nM 8.89
CHEMBL2381273 IC50 = 1.4 nM 8.85
CHEMBL2381270 IC50 = 2.6 nM 8.59
CHEMBL2381267 IC50 = 3.3 nM 8.48
CHEMBL2381261 IC50 = 3.4 nM 8.47
CHEMBL2381253 IC50 = 3.5 nM 8.46
CHEMBL2322764 IC50 = 3.9 nM 8.41
CHEMBL2381272 IC50 = 7.6 nM 8.12
CHEMBL2381262 IC50 = 8.1 nM 8.09
CHEMBL2381264 IC50 = 8.4 nM 8.08
CHEMBL2381263 IC50 = 10.2 nM 7.99
CHEMBL521851 PICTILISIB Ki = 31.0 nM 7.51
CHEMBL2381269 IC50 < 0.5 nM -
CHEMBL2381265 IC50 < 0.5 nM -
CHEMBL2381379 IC50 < 0.5 nM -