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Assay Detail

CHEMBL2383617

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]cytisine from human alpha4beta2 nAChR overexpressed in human SHEP cells after 75 mins by liquid scintillation spectrometric analysis
27
Total Activities
10
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SHEP
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907589)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Neonicotinic analogues: selective antagonists for α4β2 nicotinic acetylcholine receptors.
Faundez-Parraguez M, Farias-Rabelo N, Gonzalez-Gutierrez JP, Etcheverry-Berrios A, Alzate-Morales J, Adasme-Carreño F, Varas R, Bermudez I, Iturriaga-Vasquez P.
Bioorg Med Chem (2013) 21 : 2687 -2694
PubMed 23561269 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 7.71 9.22
IC50 10 7.14 8.92
Inhibition 7 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3 NICOTINE 4.0 2 9.22
CHEMBL2381566 3 8.92
CHEMBL2381570 3 8.09
CHEMBL2381563 3 7.95
CHEMBL2381567 2 7.81
CHEMBL2381571 3 7.65
CHEMBL2381568 3 7.63
CHEMBL2381564 3 6.82
CHEMBL2381565 3 6.66
CHEMBL2381569 2 6.34

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3 NICOTINE Ki = 0.6 nM 9.22
CHEMBL3 NICOTINE IC50 = 1.2 nM 8.92
CHEMBL2381566 Ki = 1.2 nM 8.92
CHEMBL2381566 IC50 = 4.2 nM 8.38
CHEMBL2381570 Ki = 8.1 nM 8.09
CHEMBL2381563 Ki = 11.2 nM 7.95
CHEMBL2381567 Ki = 15.5 nM 7.81
CHEMBL2381571 Ki = 22.4 nM 7.65
CHEMBL2381568 Ki = 23.4 nM 7.63
CHEMBL2381570 IC50 = 28.4 nM 7.55
CHEMBL2381563 IC50 = 39.4 nM 7.41
CHEMBL2381571 IC50 = 78.4 nM 7.11
CHEMBL2381568 IC50 = 81.9 nM 7.09
CHEMBL2381564 Ki = 151.0 nM 6.82
CHEMBL2381567 IC50 = 166.0 nM 6.78
CHEMBL2381565 Ki = 217.0 nM 6.66
CHEMBL2381569 Ki = 460.0 nM 6.34
CHEMBL2381564 IC50 = 528.0 nM 6.28
CHEMBL2381565 IC50 = 759.0 nM 6.12
CHEMBL2381569 IC50 = 1611.0 nM 5.79
CHEMBL2381563 Inhibition - % -
CHEMBL2381565 Inhibition - % -
CHEMBL2381564 Inhibition - % -
CHEMBL2381570 Inhibition - % -
CHEMBL2381566 Inhibition - % -
CHEMBL2381571 Inhibition - % -
CHEMBL2381568 Inhibition - % -