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Assay Detail

CHEMBL2393915

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of atypical PKCzeta (unknown origin) using CREBtide as substrate incubated for 1 hr measured every 2.5 mins by ADP Quest assay
9
Total Activities
9
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein kinase C zeta type (CHEMBL3438)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationships of 2-amino-3-carboxy-4-phenylthiophenes as novel atypical protein kinase C inhibitors.
Titchenell PM, Showalter HD, Pons JF, Barber AJ, Jin Y, Antonetti DA.
Bioorg Med Chem Lett (2013) 23 : 3034 -3038
PubMed 23566515 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.58 6.10
INH 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2393448 1 6.10
CHEMBL2393450 1 5.70
CHEMBL2393447 1 5.70
CHEMBL2393431 1 5.70
CHEMBL2393451 1 5.40
CHEMBL2393427 1 5.22
CHEMBL2393423 1 5.22
CHEMBL2393417 1 -
CHEMBL2393416 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2393448 IC50 = 800.0 nM 6.10
CHEMBL2393450 IC50 = 2000.0 nM 5.70
CHEMBL2393447 IC50 = 2000.0 nM 5.70
CHEMBL2393431 IC50 = 2000.0 nM 5.70
CHEMBL2393451 IC50 = 4000.0 nM 5.40
CHEMBL2393427 IC50 = 6000.0 nM 5.22
CHEMBL2393423 IC50 = 6000.0 nM 5.22
CHEMBL2393417 INH = 13.0 uM -
CHEMBL2393416 INH = 23.0 uM -