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Assay Detail

CHEMBL2393917

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of atypical PKCzeta in HEK293 cells assessed as TNF-induced NFkappaB activation incubated for 3 hrs prior to TNF induction measured after 5 hrs by bright-glo luciferase reporter gene assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein kinase C zeta type (CHEMBL3438)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationships of 2-amino-3-carboxy-4-phenylthiophenes as novel atypical protein kinase C inhibitors.
Titchenell PM, Showalter HD, Pons JF, Barber AJ, Jin Y, Antonetti DA.
Bioorg Med Chem Lett (2013) 23 : 3034 -3038
PubMed 23566515 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 8 8.68 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2393448 1 9.00
CHEMBL2393447 1 9.00
CHEMBL2393431 1 9.00
CHEMBL2393450 1 8.70
CHEMBL2393427 1 8.70
CHEMBL200027 1 8.52
CHEMBL2393423 1 8.52
CHEMBL2393451 1 8.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2393448 EC50 = 1.0 nM 9.00
CHEMBL2393447 EC50 = 1.0 nM 9.00
CHEMBL2393431 EC50 = 1.0 nM 9.00
CHEMBL2393450 EC50 = 2.0 nM 8.70
CHEMBL2393427 EC50 = 2.0 nM 8.70
CHEMBL200027 EC50 = 3.0 nM 8.52
CHEMBL2393423 EC50 = 3.0 nM 8.52
CHEMBL2393451 EC50 = 10.0 nM 8.00