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Assay Detail

CHEMBL2394837

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of pig pancreas soluble alpha-amylase using 4-nitrophenyl-alpha-D-maltopentaoside as substrate after 30 mins by spectrophotometry
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Flaxseed (Linum usitatissimum L.) extract as well as (+)-secoisolariciresinol diglucoside and its mammalian derivatives are potent inhibitors of α-amylase activity.
Hano C, Renouard S, Molinié R, Corbin C, Barakzoy E, Doussot J, Lamblin F, Lainé E.
Bioorg Med Chem Lett (2013) 23 : 3007 -3012
PubMed 23583514 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.96 5.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2391149 (+)-ENTERODIOL 1 5.07
CHEMBL368347 (+)-SECOISOLARICIRESINOL 2.0 1 5.02
CHEMBL2391150 (+)-ENTEROLACTONE 1 4.96
CHEMBL2391148 (+)-SECOISOLARICIRESINOLDIGLUCOSIDE 1 4.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2391149 (+)-ENTERODIOL IC50 = 8600.0 nM 5.07
CHEMBL368347 (+)-SECOISOLARICIRESINOL IC50 = 9500.0 nM 5.02
CHEMBL2391150 (+)-ENTEROLACTONE IC50 = 10900.0 nM 4.96
CHEMBL2391148 (+)-SECOISOLARICIRESINOLDIGLUCOSIDE IC50 = 15700.0 nM 4.80