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Assay Detail

CHEMBL2394892

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PLK1 (unknown origin) using biotin-AGAGTVPESIHSFIGDGLV as substrate by TR-FRET assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase PLK1 (CHEMBL3024)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of TAK-960: an orally available small molecule inhibitor of polo-like kinase 1 (PLK1).
Nie Z, Feher V, Natala S, McBride C, Kiryanov A, Jones B, Lam B, Liu Y, Kaldor S, Stafford J, Hikami K, Uchiyama N, Kawamoto T, Hikichi Y, Matsumoto S, Amano N, Zhang L, Hosfield D, Skene R, Zou H, Cao X, Ichikawa T.
Bioorg Med Chem Lett (2013) 23 : 3662 -3666
PubMed 23664874 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 7.98 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2392549 1 9.00
CHEMBL2392548 1 9.00
CHEMBL2392556 1 9.00
CHEMBL2392545 TAK-960 1.0 1 8.70
CHEMBL2392554 1 8.70
CHEMBL2392544 1 8.52
CHEMBL2392553 1 8.40
CHEMBL2392558 1 8.30
CHEMBL2392552 1 7.92
CHEMBL2392550 1 7.82
CHEMBL2392547 1 7.58
CHEMBL2392557 1 7.16
CHEMBL2392546 1 7.09
CHEMBL2392555 1 7.00
CHEMBL2392543 1 6.77
CHEMBL2392551 1 6.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2392549 IC50 = 1.0 nM 9.00
CHEMBL2392548 IC50 = 1.0 nM 9.00
CHEMBL2392556 IC50 = 1.0 nM 9.00
CHEMBL2392545 TAK-960 IC50 = 2.0 nM 8.70
CHEMBL2392554 IC50 = 2.0 nM 8.70
CHEMBL2392544 IC50 = 3.0 nM 8.52
CHEMBL2392553 IC50 = 4.0 nM 8.40
CHEMBL2392558 IC50 = 5.0 nM 8.30
CHEMBL2392552 IC50 = 12.0 nM 7.92
CHEMBL2392550 IC50 = 15.0 nM 7.82
CHEMBL2392547 IC50 = 26.0 nM 7.58
CHEMBL2392557 IC50 = 70.0 nM 7.16
CHEMBL2392546 IC50 = 81.0 nM 7.09
CHEMBL2392555 IC50 = 100.0 nM 7.00
CHEMBL2392543 IC50 = 170.0 nM 6.77
CHEMBL2392551 IC50 = 190.0 nM 6.72