Assay Detail

CHEMBL2395082

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding

Displacement of [3H]PGE2 from human recombinant prostanoid EP4 receptor in CHEM1 cells after 2 hrs

ChEMBL ↗

Free Research Context This assay will appear in recent viewed items on this browser. Open the demo workspace to preview project-only review queues.

Research home Demo workspace

Target

Prostaglandin E2 receptor EP4 subtype (CHEMBL1836) ↗

Open DB target context

Use one target anchor to keep assay intent, target linkage, and tested compound review aligned.

Loading context

Prostaglandin E2 receptor EP4 subtype (CHEMBL1836) is the working anchor for this assay review. Loading the live Open DB target snapshot…

Primary target Prostaglandin E2 receptor EP4 subtype

CHEMBL1836

Anchored from Assay target.

Protein identity Prostaglandin E2 receptor EP4 subtype

Disease frame Disease evidence pending

Open the live target snapshot or evidence card before reviewing assay metadata so target fit and disease framing stay reusable.

Review focus Open DB context pending

Open the live target snapshot before relying on this target frame across surface handoff.

Open target snapshot Open evidence card Search with this context

Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Binding
Organism	Homo sapiens
Cell Type	CHEM1
Confidence	9 — Direct single protein target
Curated By	Autocuration

Target

Prostaglandin E2 receptor EP4 subtype (CHEMBL1836) ↗

Type	SINGLE PROTEIN
Organism	Homo sapiens

Publication

Cinnamides as selective small-molecule inhibitors of a cellular model of breast cancer stem cells.

Germain AR, Carmody LC, Nag PP, Morgan B, Verplank L, Fernandez C, Donckele E, Feng Y, Perez JR, Dandapani S, Palmer M, Lander ES, Gupta PB, Schreiber SL, Munoz B.
Bioorg Med Chem Lett (2013) 23 : 1834 -1838
PubMed 23403082 · DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
Ki	1	9.35	9.35
IC50	1	8.96	8.96

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL548	DINOPROSTONE	4.0	2	9.35

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL548	DINOPROSTONE	Ki	=	0.45	nM	9.35
CHEMBL548	DINOPROSTONE	IC50	=	1.1	nM	8.96