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Assay Detail

CHEMBL2396023

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at CCR1 in human THP1 cells assessed as inhibition of MIP-1alpha-induced chemotaxis after 2 hrs by fluorescence assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type THP-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

C-C chemokine receptor type 1 (CHEMBL2413)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and structure activity relationships of spirocyclic compounds as potent CCR1 antagonists.
Hossain N, Ivanova S, Bergare J, Mensonides-Harsema M, Cooper ME.
Bioorg Med Chem Lett (2013) 23 : 3500 -3504
PubMed 23659855 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 8.23 9.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2332935 1 9.44
CHEMBL2332936 1 9.28
CHEMBL2391803 1 9.04
CHEMBL2391810 1 8.96
CHEMBL2391797 1 8.89
CHEMBL2391939 1 8.85
CHEMBL2391795 1 8.85
CHEMBL2391808 1 8.74
CHEMBL2391793 1 8.74
CHEMBL2391804 1 8.72
CHEMBL2391800 1 8.60
CHEMBL2391938 1 8.60
CHEMBL2391937 1 8.55
CHEMBL2391794 1 8.49
CHEMBL2391799 1 8.30
CHEMBL2391809 1 8.15
CHEMBL2391806 1 8.10
CHEMBL2391796 1 8.10
CHEMBL2391798 1 8.05
CHEMBL2391802 1 7.92
CHEMBL2391812 1 7.92
CHEMBL2391811 1 7.50
CHEMBL2391935 1 7.15
CHEMBL2391807 1 7.05
CHEMBL2391805 1 6.96
CHEMBL2391801 1 6.89
CHEMBL2391936 1 6.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2332935 IC50 = 0.36 nM 9.44
CHEMBL2332936 IC50 = 0.52 nM 9.28
CHEMBL2391803 IC50 = 0.92 nM 9.04
CHEMBL2391810 IC50 = 1.1 nM 8.96
CHEMBL2391797 IC50 = 1.3 nM 8.89
CHEMBL2391939 IC50 = 1.4 nM 8.85
CHEMBL2391795 IC50 = 1.4 nM 8.85
CHEMBL2391808 IC50 = 1.8 nM 8.74
CHEMBL2391793 IC50 = 1.8 nM 8.74
CHEMBL2391804 IC50 = 1.9 nM 8.72
CHEMBL2391800 IC50 = 2.5 nM 8.60
CHEMBL2391938 IC50 = 2.5 nM 8.60
CHEMBL2391937 IC50 = 2.8 nM 8.55
CHEMBL2391794 IC50 = 3.2 nM 8.49
CHEMBL2391799 IC50 = 5.0 nM 8.30
CHEMBL2391809 IC50 = 7.1 nM 8.15
CHEMBL2391806 IC50 = 7.9 nM 8.10
CHEMBL2391796 IC50 = 7.9 nM 8.10
CHEMBL2391798 IC50 = 8.9 nM 8.05
CHEMBL2391802 IC50 = 12.0 nM 7.92
CHEMBL2391812 IC50 = 12.0 nM 7.92
CHEMBL2391811 IC50 = 32.0 nM 7.50
CHEMBL2391935 IC50 = 71.0 nM 7.15
CHEMBL2391807 IC50 = 89.0 nM 7.05
CHEMBL2391805 IC50 = 110.0 nM 6.96
CHEMBL2391801 IC50 = 130.0 nM 6.89
CHEMBL2391936 IC50 = 400.0 nM 6.40