Assay Detail
Functional
CHEMBL2396137
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at serotonin-activated human recombinant 5HT-2C receptor expressed in HEK293 cells assessed as increase in intracellular calcium level after 5 mins measured for 1 min by fluorescence assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Novel pyrimidoazepine analogs as serotonin 5-HT(2A) and 5-HT(2C) receptor ligands for the treatment of obesity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 5 | 6.34 | 8.09 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL478 | CHLOROPHENYLPIPERAZINE | 2.0 | 1 | 8.09 |
| CHEMBL2393233 | — | — | 1 | 5.92 |
| CHEMBL2393235 | — | — | 1 | 5.02 |
| CHEMBL2393236 | — | — | 1 | - |
| CHEMBL2393232 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL478 | CHLOROPHENYLPIPERAZINE | EC50 | = | 8.1 | nM | 8.09 |
| CHEMBL2393233 | — | EC50 | = | 1200.0 | nM | 5.92 |
| CHEMBL2393235 | — | EC50 | = | 9600.0 | nM | 5.02 |
| CHEMBL2393236 | — | EC50 | > | 10000.0 | nM | - |
| CHEMBL2393232 | — | EC50 | > | 10000.0 | nM | - |