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Assay Detail

CHEMBL2400839

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]SP1-7 from substance P receptor (1 to 7 amino acids) binding site in Sprague-Dawley rat spinal cord membranes after 60 mins by liquid scintillation counting analysis
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Substance-P receptor (CHEMBL4027)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Constrained H-Phe-Phe-NH2 analogues with high affinity to the substance P 1-7 binding site and with improved metabolic stability and cell permeability.
Fransson R, Sköld C, Kratz JM, Svensson R, Artursson P, Nyberg F, Hallberg M, Sandström A.
J Med Chem (2013) 56 : 4953 -4965
PubMed 23735006 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 7.63 8.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2396664 1 8.66
CHEMBL2397494 1 8.48
CHEMBL2397492 1 8.21
CHEMBL108438 1 8.08
CHEMBL2397484 1 8.03
CHEMBL2397493 1 7.94
CHEMBL2397490 1 7.76
CHEMBL2397485 1 7.58
CHEMBL2397487 1 7.47
CHEMBL2397488 1 7.30
CHEMBL2397491 1 7.17
CHEMBL2397483 1 7.15
CHEMBL2397489 1 7.03
CHEMBL2397486 1 6.87
CHEMBL2397482 1 6.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2396664 Ki = 2.2 nM 8.66
CHEMBL2397494 Ki = 3.3 nM 8.48
CHEMBL2397492 Ki = 6.2 nM 8.21
CHEMBL108438 Ki = 8.4 nM 8.08
CHEMBL2397484 Ki = 9.4 nM 8.03
CHEMBL2397493 Ki = 11.5 nM 7.94
CHEMBL2397490 Ki = 17.5 nM 7.76
CHEMBL2397485 Ki = 26.0 nM 7.58
CHEMBL2397487 Ki = 33.9 nM 7.47
CHEMBL2397488 Ki = 50.5 nM 7.30
CHEMBL2397491 Ki = 68.0 nM 7.17
CHEMBL2397483 Ki = 70.4 nM 7.15
CHEMBL2397489 Ki = 93.1 nM 7.03
CHEMBL2397486 Ki = 136.0 nM 6.87
CHEMBL2397482 Ki = 189.0 nM 6.72